Fig. 1: Mst1/2 are indispensable for early NK cell development.

A Flow cytometry analysis (left) and mean fluorescence intensity (MFI, right) of Mst1 levels in NKp (CD3⁻CD122⁺CD11b⁻NK1.1⁻), immature NK (CD3⁻CD122⁺CD11b⁻NK1.1⁺, imNK) and mature NK (CD3⁻CD122⁺CD11b⁺NK1.1⁺, mNK) cells from the spleen of wild-type mice (WT) (n = 4). B Flow cytometry analysis (left) and MFI intensity (right) of Mst1 levels in CD27 SP (CD3⁻NKp46⁺CD27⁺CD11b⁻), DP (CD3⁻NKp46⁺CD27⁺CD11b⁺) and CD11b SP (CD3⁻NKp46⁺CD27⁻CD11b⁺) NK cells from the spleen of WT mice (n = 4). C Flow cytometry analysis (left) and MFI intensity (right) of p-Mob1 expression in splenic NK cells before and after stimulation with 50 ng/mL IL-15 for 30 min (n = 4). D–F Flow cytometry analysis of CD3⁻NK1.1⁺ NK cells in bone marrow (BM) and spleen from WT and the indicated conditional knockout mice (D). The percentages (E) and the absolute numbers (F) of NK cells in spleen and BM from the indicated mice (n = 5). The percentages of NKp, imNK, mNK cells on gated CD3⁻CD122⁺ cells (G) and CD27 SP, DP, CD11b SP NK cell subsets on gated CD3⁻NKp46⁺ cells (H) in BM (left) and spleen (right) from the indicated mice (n = 5). I Percentages of CD127⁺ (left) and KLRG1⁺ (right) NK cells in the BM and spleen from the Mst1/2^fl/fl and Mst1/2^fl/flCD122^Cre mice (n = 3). J Representative plots (left) and summary data (right) of the percentage of NK cells in the BM and spleen from BM chimera mice (n = 5). Data of A–I are representative of three independent experiments with similar results. Data of J are representative of two independent experiments with similar results.