Fig. 5: Mst1/2 control mitochondrial metabolism and redox homeostasis in NK cells.

Seahorse extracellular flux analysis was performed to measure the oxygen consumption rate (OCR) in NK cells from the BM (A) and spleen (B) of Mst1/2^fl/fl and Mst1/2^fl/flCD122^Cre mice. Flow cytometry analysis (left) and MFI intensity (right) illustrate the expression levels of mitoTracker (C, D), TMRM (E, F), total cellular ROS (G, H), mitochondrial ROS (I, J) and p-S6 (K, L) in NK cells from BM (C, E, G, I, K) and spleen (D, F, H, J, L) of Mst1/2^fl/fl and Mst1/2^fl/flCD122^Cre mice (n ≥ 4). M Representative flow cytometry plot (left) and summary data (right) display the percentage of dead cells in spleen from Mst1/2^fl/fl and Mst1/2^fl/flCD122^Cre mice fed water with or without NAC (1.5 g/L) for 30 days starting at 21-day-old (n ≥ 4). N Representative flow cytometry plot (left) and summary data (right) show the number of CD3⁻CD122⁺ cells in spleen from Mst1/2^fl/fl and Mst1/2^fl/flCD122^Cre mice fed water with or without NAC (1.5 g/L) for 30 days starting at 21-day-old (n ≥ 4). Data of C–L are representative of three independent experiments with similar results. Data of M and N are representative of two independent experiments with similar results.