Fig. 2: Effects of ADT-OH on the formation of pseudopodia and the cell apoptosis, cell cycle. | Cell Death & Disease

Fig. 2: Effects of ADT-OH on the formation of pseudopodia and the cell apoptosis, cell cycle.

From: ADT-OH exhibits anti-metastatic activity on triple-negative breast cancer by combinatorial targeting of autophagy and mitochondrial fission

Fig. 2

A The cell apoptosis assay was determined with Annexin V/PI staining using flow cytometry analysis. MDA-MB-231, 4T1-Luci and MCF-7 breast cancer cells were treated with ADT-OH at different concentration (0–50 μM), and collected at 48 h after treatment for annexin V and propidium iodide double staining. B Cell cycle progression was determined with PI staining using flow cytometry analysis. C, D Quantitative analyses of apoptosis and cell cycle were determined as mean ± SD for triplicate experiments. Differences between treatment and control were statistically analyzed using Student’s t test (NS not significant, *P < 0.1 **P < 0.01, ***P < 0.005). E Cells were stained with Alexa Fluor 488 phalloidin for F-actin (green) and DAPI for Nuclei (blue), and then visualized with Zeiss confocal microscope. The quantitative analysis of cytoskeletal staining is presented in Supplementary Fig. S1. Scale bar, 25 μm; scale bar in enlarged image, 12.5 μm.

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