Fig. 6: Effects of ADT-OH on mitophagy in MDA-MB-231 and 4T1 cells. | Cell Death & Disease

Fig. 6: Effects of ADT-OH on mitophagy in MDA-MB-231 and 4T1 cells.

From: ADT-OH exhibits anti-metastatic activity on triple-negative breast cancer by combinatorial targeting of autophagy and mitochondrial fission

Fig. 6: Effects of ADT-OH on mitophagy in MDA-MB-231 and 4T1 cells.

A Representative TEM images of control and 50 μM ADT-OH treated breast cancer cells. Black arrowheads in panel (A) indicate autophagy, red arrowheads indicate mitophagy. B Representative colocalization images of mitochondria (MitoTracker Red) and autophagosomes (GFP-LC3B). Quantitative analysis of LC3 puncta - mitochondria colocalization per cell (colocalization area: LC3 puncta area) is presented in Supplementary Fig. S4B. For each picture, an enlargement of the merged image is displayed and the white arrowheads in the enlarged view of the white box highlight colocalization of mitochondria and autophagosome. Scale bars, 15 μm; scale bar in enlarged image, 7.5 μm.

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