Fig. 4: Transcriptomic response of muscle fibers to obesity.

A, B GO analysis showing enriched biological processes and molecular function terms of DEGs between EDL and SOL from Ob/Ob mice. C GSEA analysis of amino acid import across plasma membrane. D GSEA analysis of immune response. E Venn diagram illustrating the genes commonly expressed in EDL and SOL, as well as the DEG specific to EDL and SOL in Ob/Ob mice. F Rcan1 and Mettl21c are the only two genes that exhibit opposite expression patterns within the intersection of DEGs in Ob/Ob EDL and Ob/Ob SOL. G The top 10 genes exhibiting the most significant expression changes between Ob/Ob EDL and Ob/Ob SOL, with FPKM > 10 in either muscle. H Fold change of calcium-related genes in Ob/Ob EDL and Ob/Ob SOL; the left box represents the fold change of relevant genes between control and Ob/Ob EDL, and the right box represents genes in SOL. Data are expressed as the mean ± SEM; n = 3; ns indicated not significant, ^*P < 0.05, ^**P < 0.01, ^****P < 0.0001.