Fig. 1: Coronin 2B specifically localizes at the nucleolus in neurons.
From: Coronin 2B deficiency induces nucleolar stress and neuronal apoptosis
A–D Coronin 2B expression is enriched in the nucleus in neurons. Cytoplasmic and nuclear fractions were isolated from cortical neurons cultured for 14 days (A, B) and rat brain tissues (C, D). Western blot analysis was performed using antibodies against coronin 2B; the nucleolar markers, UBF, FBL, and B23; and the cytoplasmic marker, GAPDH (A, C). Coronin 2B was quantified by immunoblotting and normalized to that of GAPDH and FBL (B, D). Data are mean ± SEM (from three independent experiments, unpaired Student’s t-test, *p < 0.05). Nuc: nucleolar, Cyto: cytoplasmic. E Coronin 2B localizes at the nucleolus. Dissociated hippocampal neurons were cultured for 14 days and then immunostained with anti-coronin 2B (green) and antibodies against the nucleolar markers, UBF, FBL, and B23 (red). Scale bars: 5 µm. F Morphological variation during different development stages of the nucleolus. DIV days in vitro. Scale bars: 5 µm.
