Fig. 5: The neurodegeneration transcription program is activated upon coronin 2B depletion.
From: Coronin 2B deficiency induces nucleolar stress and neuronal apoptosis
A Volcano plot of differentially expressed genes (i.e., p < 0.01 and log2[fold-change] >1 or <−1). Vertical and horizontal dotted lines represent the threshold of −log10(adjusted p values) and log2(fold-change). B Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis of variable transcripts (cutoff: p < 0.01). C Validation of the expression levels of genes related to the indicated pathways from RNA sequencing analysis by qPCR. RNA was extracted from cortical primary neurons cultured for 9 days and transduced with shCoro2B lentivirus or corresponding scramble control. Then, qPCR was performed using primers for selected genes. Data were mean ± SEM (from three independent experiments; unpaired t-test with Welch’s correction; *p < 0.05, **p < 0.01, ***p < 0.001). D Heatmap of p53 pathway-related gene expression from RNA sequencing analysis. E RT-qPCR analysis of the top five genes with the greatest variable expression in the p53 pathway. Data were mean ± SEM (from three independent experiments; unpaired t-test with Welch’s correction; *p < 0.05, **p < 0.01, ***p < 0.001).
