Fig. 2: Khsrp protects against ALF in vivo. | Cell Death & Disease

Fig. 2: Khsrp protects against ALF in vivo.

From: KHSRP ameliorates acute liver failure by regulating pre-mRNA splicing through its interaction with SF3B1

Fig. 2

A–C Expression of KHSRP protein was detected using (A) immunohistochemistry and (C) Western blotting in the mouse livers (n = 6) following treatment with APAP or CCl4 for 24 h (scale bar: 50 μm). B The graph shows KHSRP protein levels in (A) as mean ± SEM. D Expression of Khsrp mRNA was detected in the livers induced by APAP or CCl4 for 24 h using real-time PCR. E Schematic diagram of the experimental setup. Mice (n = 6) were first infected with either AAV-shKhsrp or AAV8-Khsrp for 2 weeks using tail-vein injections. They were then intraperitoneally injected with CCl4 or APAP for an additional 24 h. F–M Expression of KHSRP protein was detected in the injured livers after (F) AAV-shKhsrp and (J) AAV8-Khsrp injection using Western blotting. The graph shows KHSRP protein levels as means ± SEM. H&E staining of liver sections from (G) Khsrp-knockdown and (K) Khsrp-overexpressing mice following treatment with CCl4 or APAP (scale bars: 100 μm). H and L Necrosis quantification is shown in the lower panels. (I and M) Plasma ALT and AST levels in mice. Data represent means ± SEM from three independent experiments. *p < 0.05.

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