Fig. 5: Impaired EC barrier, increased blood vessel density, and increased BBB leakage in NEO1GFAP-Cre cortex. | Cell Death & Disease

Fig. 5: Impaired EC barrier, increased blood vessel density, and increased BBB leakage in NEO1GFAP-Cre cortex.

From: Hepcidin depending on astrocytic NEO1 ameliorates blood-brain barrier dysfunction after subarachnoid hemorrhage

Fig. 5: Impaired EC barrier, increased blood vessel density, and increased BBB leakage in NEO1GFAP-Cre cortex.

A Representative images of EB (red) coimmunostained with PECAM-1 antibody in NEO1fl/fl and NEO1GFAP-Cre cortex (Imaged at P30). B, C Quantitative analyses of BV length (B) and EB extravasation (C). D, E Representative images of claudin-5 (D) and ZO-1 (E) coimmunostained with PECAM-1 antibody in NEO1fl/fl and NEO1GFAP-Cre cortex. F, G Quantitative analyses of data in (D) and (E). H, I Representative images of PLVAP (H) and caveolin-1 (I) coimmunostained with PECAM-1 antibody in NEO1fl/fl and NEO1GFAP-Cre cortex. PLVAP is a maker for EC fenestrae, caveolin-1 is an EC caveolae marker. J, K Quantitative analyses of data in (H) and (I). Scale bars: 20 μm. **P < 0.01; ns not significant. Student’s t-test.

Back to article page