Fig. 2: BUB1b regulated the viability of LUAD cells in vitro and in vivo.
From: BUB1b impairs chemotherapy sensitivity via resistance to ferroptosis in lung adenocarcinoma
A, B The expression of BUB1b in five LUAD cell lines (A549, H1299, H1395, H1975, and H460) and HBE was detected by immunoblotting (A); the mRNA level of BUB1b in five LUAD cell lines (A549, H1299, H1395, H1975, and H460) and HBE was detected via qRT-PCR (B). C The transfection efficiency of lentivirus silencing or overexpressing BUB1b was verified by immunoblotting in A549 and H460 cells. D The knockdown of BUB1b in A549 cells with lentivirus was measured by qRT-PCR. E The overexpression of BUB1b in H460 cells was confirmed by qRT-PCR. F, G After the silencing of BUB1b in A549 cells and overexpression of BUB1b in H460 cells, the viability was evaluated by CCK-8 assays (F) and colony formation assays (G). H A549-NC, A549-shBUB1b-1, A549-shBUB1b-2, H460-vector, and H460-BUB1b were subcutaneously implanted into BALB/c nude mice. The tumor size (volume = 1/2 width2 × length) was recorded every three days. After the mice were sacrificed, the tumors were resected and weighed. I Kaplan‒Meier plots comparing the survival profiles of nude mice bearing tumors derived from A549-NC, A549-shBUB1b-1, A549-shBUB1b-2, H460-vector, and H460-BUB1b cells. Student’s t-test and one-way ANOVA were applied to compare the differences. *p < 0.05, **p < 0.01, and ***p < 0.001.
