Fig. 1: DKK1-SE locus exhibits high activity within PDAC. | Cell Death & Disease

Fig. 1: DKK1-SE locus exhibits high activity within PDAC.

From: DKK1-SE recruits AP1 to activate the target gene DKK1 thereby promoting pancreatic cancer progression

Fig. 1

A H3K27ac ChIP-seq tracks on DKK1-SE locus. Pancreas represents normal pancreatic tissue and K562, HCC827, T24, and CAL51 represent DKK1-SE negative cells. B ChIP-qPCR analysis of H3K27ac on DKK1-SE in PANC-1, HPAC and AsPC-1 cells, which used PGL4.10 vector as negative control. C Heatmap of chromatin interactions and TAD structural domains near the DKK1-SE locus in PANC-1 and pancreas. D The interaction frequency between DKK1-SE and adjacent locus were quantified, taking DKK1-SE as the viewpoint. Viewpoints are indicated by black vertical line anchored to their genomic locations. Interaction frequency data were visualized using wash u epigenome browser (http://epigenomegateway.wustl.edu/). E ChIA-PET and IM-PET interactions data of DKK1-SE with DKK1 promoter region and LINC-AROD promoter region. Data from 4DGenome database (4dgenome.research.chop.edu). F The mRNA expression levels of DKK1 and LINCA-ROD in PANC-1, HPAC, and AsPC-1 after treated with 500 nM JQ1 by qRT-PCR. G The mRNA expression levels of DKK1 and LINCA-ROD in PANC-1, HPAC, and AsPC-1 after treated with 50 pM siBRD4 by qRT-PCR. The qRT-PCR data were normalized to the expression of GAPDH. Means of three biological replicates are shown. Error bars indicate SEMs. **P < 0.01; ***P < 0.001; ns. no significance by two-tailed Student’s t test.

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