Fig. 5: DKK1-SE influences DKK1 promoter activity via AP1 TFs. | Cell Death & Disease

Fig. 5: DKK1-SE influences DKK1 promoter activity via AP1 TFs.

From: DKK1-SE recruits AP1 to activate the target gene DKK1 thereby promoting pancreatic cancer progression

Fig. 5

A ChIP-qPCR analysis of H3K27ac、H3K4me1、H3K27me3、EZH2 on DKK1-SE (e1) after treated with 10 pM SR11302. B ChIP-qPCR analysis of H3K27ac、H3K4me1、H3K27me3、EZH2 on DKK1 promoter after deletion of DKK1-SE or treated with 10 pM SR11302. IgG were used as negative control in A and B. C Methylation modifications of the DKK1 promoter after deletion of DKK1-SE or treated with 10 pM SR11302. Hollow circles represent CpG islands in a hypomethylated state and solid circles represent CpG islands in a hypermethylated state. D A proposed working model for the function of AP1 in regulating chromatin looping between enhancer and promoter regions. Means of three biological replicates are shown. Error bars indicate SEMs. *P < 0.05; **P < 0.01; ***P < 0.001; ns. no significance by two-tailed Student’s t test.

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