Table 3 sgRNAs for dCas-KRAB and CRISPR/Cas9.
sgRNAs for dCas-KRAB | ||||
|---|---|---|---|---|
Region | Primer (5′ - 3′) | Primer | Location (HG38) | Target site sequence (with PAM) |
DKK1_1.1 | Forward | CACC GGTCAGGACTCTGGGACCGCAG | chr10:52314304-52314326 | TCAGGACTCTGGGACCGCAG GGG |
Reverse | AAAC CTGCGGTCCCAGAGTCCTGA CC | |||
DKK1_1.2 | Forward | CACC GGCCCAGAGCCATCATCTCAGA | chr10:52314426-52314448 | CCCAGAGCCATCATCTCAGA AGG |
Reverse | AAAC TCTGAGATGATGGCTCTGGG CC | |||
e1.1 | Forward | CACC GGATTATAAAATAGACCCTCTG | chr10:52443485-52443507 | ATTATAAAATAGACCCTCTG GGG |
Reverse | AAAC CAGAGGGTCTATTTTATAAT CC | |||
e1.2 | Forward | CACC GGGGAATGTTTACTGGTCTACC | chr10:52443928-52443950 | GGAATGTTTACTGGTCTACC AGG |
Reverse | AAAC GGTAGACCAGTAAACATTCC CC | |||
e2.1 | Forward | CACC GGGTAAACCAATTCTCCTCACA | chr10:52473103-52473125 | GTAAACCAATTCTCCTCACA TGG |
Reverse | AAAC TGTGAGGAGAATTGGTTTAC CC | |||
e2.2 | Forward | CACC GGAACAGTTGGAAGGATTGGAA | chr10:52472554-52472576 | AACAGTTGGAAGGATTGGAA AGG |
Reverse | AAAC TTCCAATCCTTCCAACTGTT CC | |||
e3.1 | Forward | CACC GGCAGCTGTGGTTTGGAGTGAA | chr10:52486965-52486987 | CAGCTGTGGTTTGGAGTGAA AGG |
Reverse | AAAC TTCACTCCAAACCACAGCTG CC | |||
e3.2 | Forward | CACC GGCTTCGCTGATAAGCAGACCT | chr10:52487116-52487138 | CTTCGCTGATAAGCAGACCT AGG |
Reverse | AAAC AGGTCTGCTTATCAGCGAAG CC | |||
e4.1 | Forward | CACC GGGACTCTCTTTCTCCACCTGG | chr10:52489073-52489095 | GACTCTCTTTCTCCACCTGG TGG |
Reverse | AAAC CCAGGTGGAGAAAGAGAGTC CC | |||
e4.2 | Forward | CACC GGTTTTTAAAATTCTAACCCTA | chr10:52489025-52489047 | TTTTTAAAATTCTAACCCTA GGG |
Reverse | AAAC TAGGGTTAGAATTTTAAAAA CC | |||
sgRNAs for CRISPR/Cas9 | ||||
|---|---|---|---|---|
ko_up | Forward | CACC GGTAGTGGACACATGTGTGGCG | chr10:52442298-52442320 | TAGTGGACACATGTGTGGCG GGG |
Reverse | AAAC CGCCACACATGTGTCCACTA CC | |||
ko_down | Forward | CCGG TTATTAATCTAAGTTATCTC | chr10:52444362-52444384 | TTATTAATCTAAGTTATCTC AGG |
Reverse | AAAC GAGATAACTTAGATTAATAA | |||
