Table 4 Primers for PCR identified monoclonal genotype.
Primers for PCR identified homozygous clones | ||||
|---|---|---|---|---|
Region | Primer (5′ - 3′) | Primer | Location (HG38) | Size (bp) |
f1 | Forward | GATGATCCTGCAGATGTCCA | chr10:52442048-52444588 | 2541 |
Reverse | TCATCATAGGTCTATGCACCAA | |||
f2 | Forward | GCTGGCTCATGCTAGCGAA | chr10:52443534-52443758 | 225 |
Reverse | TGCTATGAATCAGGCACCACA | |||
Primers for off-target detection of CRISPR/Cas9 | ||||
|---|---|---|---|---|
Region | Primer (5′ - 3′) | Primer | Location (HG38) | Size (bp) |
off-target site1-1 | Forward | GTGAGGAATCCGTGGATAAA | chr8:73136566-73136588 | 442 |
Reverse | GGCAGGCAGACAGTAAACAG | |||
off-target site1-2 | Forward | TCTTGATATGCTGCACAAACA | chrX:135147974-135148596 | 422 |
Reverse | AGATAGGCCACAATGACCAC | |||
off-target site1-3 | Forward | AGATAGGCCACAATGACCAC | chrX:135223948-135224570 | 419 |
Reverse | TGATATGCTGCACAAACACC | |||
off-target site2-1 | Forward | AAGGAAAAGCAAGTGAGAAG | chr20:1795603-1796225 | 390 |
Reverse | TAAAATGAGTTGGGAAGTGT | |||
off-target site2-2 | Forward | GGCACTTACAATACACCTAA | chr6:118013776-118014398 | 485 |
Reverse | AGAGCCCACCATTTCTCACA | |||
off-target site2-3 | Forward | GATTTGTTTGGAATGAGGAA | chr13:75921023-75921645 | 391 |
Reverse | TAGAAATCACCAAATAGCCT | |||
