Fig. 7: IL-6 was the key downstream molecule of AQP3-mediated tumor progression. | Cell Death & Disease

Fig. 7: IL-6 was the key downstream molecule of AQP3-mediated tumor progression.

From: PPAR-γ/NF-kB/AQP3 axis in M2 macrophage orchestrates lung adenocarcinoma progression by upregulating IL-6

Fig. 7

A Cytokine antibody microarray image displaying the expression of cytokines in the supernatant of mouse-derived macrophages with AQP3 knockdown. B The cytokine antibody chip membrane showing different spots corresponding to different cytokines. C RT-qPCR analysis of cytokine expression in LLC cells after co-culture with macrophages. D, E ELISA analysis of IL-6 expression in AQP3-knockdown M2 macrophages and co-cultured LLC cells. F Western blot analysis of IL-6 and IL-6R protein expressions under different concentrations of IL-6 stimulation. G Western blot analysis of IL-6, IL-6R, STAT3 and p-STAT3 protein expressions in LLC cells co-cultured with si-AQP3 and IL-6 intervention. H, I Immunofluorescence staining of IL-6 and IL-6R fluorescence intensity in LLC cells co-cultured with si-AQP3 and IL-6 intervention. J, K G6PDH and LDH activity assays detecting glucose metabolism in LLC cells co-cultured with si-AQP3 and IL-6 intervention. POS: positive controls; NEG: negative controls. **P < 0.01, ***P < 0.001, ****P < 0.0001.

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