Table 1 Real-time PCR primers to quantitate the expression of Shp2, Gab1, Occludin, and inflammatory markers.

From: Age-related retinal degeneration resulting from the deletion of Shp2 tyrosine phosphatase in photoreceptor neurons

Gene

Forward Primer

Reverse Primer

Shp2

CCATGGGCAGCTGAAAGAGA

TACGAGTTGTGTTGAGGGGC

Gab1

CTCCGGAGGAGTTTCAGAGAA

TTCGCGGACTGAAGAGGAAC

Occludin

TTGAAAGTCCACCTCCTTACAGA

CCGGATAAAAAGAGTAGCCTGG

Rpl37

CGGGACTGGTCGGATGAG

TCACGGAATCCATGTCTGAATC

Actb (β-actin)

ACTGGGACGACATGGAGAAG

GGGGTGTTGAAGGTCTCAAA

Arg1

GAACACGGCAGTGGCTTTAAC

TGCTTAGCTCTGTCTGCTTTGC

Cd206

TCTTTGCTTTCCAGTCTCC

TGACACCCAGCGGAATTTC

Cd32

AATCCTGCCGTTCCTACTGATC

GTGTCACCGTGTCTTCCTTGAG

Gfap

CCAGCTTACGGCCAACAGT

TGGTTTCATCTTGGACTTCTG

Il-8

CCTGCTCTGTCACCGATG

CAGGGCAAAGAACAGGTCAG

Il-10

ACCTGCTCCACTGCCTTGCT

GGTTGCCAAGCCTTATCGGA

Il-1β

TCGAGGCCTAATAGGCTCATCT

GCTGCTTCAGACACTTGCACAA

Ifn-γ

TGGCTCTGAAGGATTTTCATC

TCAACTGGCATAGATGTGGAAGAA

Inos

CATTGGAAGTGAAGCGTTTCG

CAGCTGGGCTGTACAAACCTT

Mcp1

AAGTGCAGAGAGCCAGACG

TCAGTGAGAGTTGGCTGGTG

Nfh

CACCAAGGAGTCACTGGAG

TGCTGAATAGCGTCCTGGTA

Tnf-α

CCTCTTCTCATTCCTGCTTGTGG

GGCCATTTGGGAACTTCTCATC

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