Fig. 2: Elevated HMGA1 promotes malignant phenotype of ESCC cells. | Cell Death & Disease

Fig. 2: Elevated HMGA1 promotes malignant phenotype of ESCC cells.

From: HMGA1 promotes the progression of esophageal squamous cell carcinoma by elevating TKT-mediated upregulation of pentose phosphate pathway

Fig. 2

A–C HMGA1 knockdown inhibits the proliferation of ESCC cells. HMGA1 was knocked down in KYSE30 cells. Western blot was performed to detect the efficiency of the knockdown (A). Cell proliferation of the control and shHMGA1 cells was detected by colony formation assay (B). Colonies in (B) were calculated and shown in (C), n = 3. D–F Subcutaneous syngeneic tumors generated from HMGA1-knocked down mouse esophageal cancer AKR cells, n = 6. D Tumors, E Tumor weight, F Tumor volume. Tumor length (L) and width (W) were measured using vernier calipers every other day from day 3 after transplantation. The tumor volume was calculated using the formula (L × W2)/2 and presented as mean ± SEM. G–I HMGA1 overexpression promotes the proliferation of ESCC cells. A stabe cell line with HMGA1 overexpression was established in TE13 cells. Western blot was performed to detect the efficiency of the enforced expression (G). Cell proliferation of the control and HMGA1 overexpression cells was detected by colony formation assay (H). Colonies in (H) were calculated and shown in (I), n = 3. J–L Tumors and their weight and volume of subcutaneous syngeneic tumors generated from HMGA1-overexpressed mouse esophageal cancer AKR cells. Tumor establishment and measurement were performed as described in (D–F), n = 6. *P < 0.05, **P < 0.01, and ***P < 0.001.

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