Fig. 4: HMGA1 upregulates TKT expression. | Cell Death & Disease

Fig. 4: HMGA1 upregulates TKT expression.

From: HMGA1 promotes the progression of esophageal squamous cell carcinoma by elevating TKT-mediated upregulation of pentose phosphate pathway

Fig. 4

A RNA-seq was performed in control and HMGA1-silenced KYSE30 cells. Heatmap of partially differentially expressed genes in cell metabolism in HMGA1-silenced cells versus control cells. B Correlation analysis between the HMGA1 and TKT expression in ESCCs using online TCGA database. C Representative IHC staining of TKT in paired ESCC tissues (Scale bars: 50 μm). D Representative IHC staining of TKT in 4NQO-induced esophageal tumor tissue (Scale bars: 20 μm). E The tSNE map showed expression of TKT in ESCC tumor tissues from scRNA-seq database (GSE188900 dataset). F, G A stable cell line with HMGA1 overexpression was established in TE13 cells. Western blot (F) was performed to detect the expression of TKT and γ-H2AX in HMGA1-overexpressed TE13 cells exposing to CDDP (10 μM) for 16 h. The enzymatic activity (G) of TKT was measured in HMGA1-overexpressed TE13 cells. H TKT in esophageal cancers induced by 4NQO in HMGA1KI/KI (control) and HMGA1KI/KIK14-cre+ mice. HMGA1 gene knock-in (HMGA1KI/KIK14-cre+) and its control (HMGA1KI/KI) mice were treated with 80 mg/L 4NQO in drinking water for 5 months for the induction of esophageal cancer (n = 6). Representative images of esophageal sections stained with HMGA1 and TKT antibody are shown. Scale bars, 50 μm. I, J A stable cell line with HMGA1-knocked down was established in KYSE30 cells. Western blot (I) was performed to detect the expression of TKT and γ-H2AX in HMGA1-knocked down KYSE30 cells exposed to CDDP (10 μM) for 16 h. The enzymatic activity (J) of TKT was measured in HMGA1-knocked down KYSE30 cells. K TKT in esophageal cancers induced by 4NQO in HMGA1flox/flox and HMGA1flox/floxK14 mice. Conditional HMGA1 gene knock-out in esophagus (HMGA1flox/floxK14) and its control (HMGA1flox/flox) mice were treated with 80 mg/L 4NQO in drinking water for 5 months for the induction of esophageal cancer (n = 6). Representative images of esophageal sections stained with HMGA1 and TKT antibody are shown. Scale bars, 50 μm. Student’s t test was used for the statistical analysis. *P < 0.05, n = 3.

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