Fig. 1: MTA1 is upregulated in breast cancer and promotes the proliferation of breast cancer cells. | Cell Death & Disease

Fig. 1: MTA1 is upregulated in breast cancer and promotes the proliferation of breast cancer cells.

From: The feedback loop between MTA1 and MTA3/TRIM21 modulates stemness of breast cancer in response to estrogen

Fig. 1: MTA1 is upregulated in breast cancer and promotes the proliferation of breast cancer cells.

A Analysis of MTA family expression using the GEO dataset indicated that MTA1 was increased and MTA3 was decreased in breast cancer samples compared to normal tissues. B Survival analysis demonstrated that high expression of MTA1 indicates a good prognosis, and high expression of MTA3 indicates a poor prognosis. C MTA1 expression was negatively correlated with MTA3 expression using the analysis of public datasets. D The expression of MTA1 and MTA3 was analyzed by qRT-PCR using total RNA extracted from paired samples of 12 breast cancer tissues and adjacent normal breast tissues. E Lysates of different human breast cancer cell lines were subjected to western blotting to examine MTA1 and MTA3 protein expression levels. F Growth curve analysis of MCF-7 and MDA-MB-231 cells after overexpression or knockdown of MTA1 or MTA3. G EdU assays in MCF-7 and MDA-MB-231 cells transfected with vector, MTA1, MTA3, shSCR, shMTA1, or shMTA3. H Colony formation experiments after transfection with vector, MTA1, MTA3, shSCR, shMTA1, and shMTA3 in MCF-7 and MDA-MB-231. Error bars represent the minimum to maximum in A and the mean ± SD in D, F–H. *p < 0.05, **p < 0.01, ***p < 0.001; two-tailed unpaired t-test.

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