Fig. 2: MTA1 promotes migration, invasion, EMT, and stemness in breast cancer cells, whereas MTA3 exerts the opposite effects. | Cell Death & Disease

Fig. 2: MTA1 promotes migration, invasion, EMT, and stemness in breast cancer cells, whereas MTA3 exerts the opposite effects.

From: The feedback loop between MTA1 and MTA3/TRIM21 modulates stemness of breast cancer in response to estrogen

Fig. 2: MTA1 promotes migration, invasion, EMT, and stemness in breast cancer cells, whereas MTA3 exerts the opposite effects.

A Transwell invasion assays performed in MCF-7 and MDA-MB-231 cells after overexpression or knockdown of MTA1 or MTA3, respectively. B mRNA and protein expression levels of epithelial and mesenchymal markers, as determined by RT-qPCR and western blotting, respectively, after overexpression of MTA1 or knockdown of MTA3 in MCF-7 cells, knockdown of MTA1, or overexpression of MTA3 in MDA-MB-231 cells. C Representative images of spheroid formation in suspension culture after 15 days of MTA1 overexpression or MTA3 knockdown in MCF-7 cells. The number of spheres per field and the diameters of the spheres were statistically analyzed. D Flow cytometry results of EpCAM-stained MDA-MB-231 cells stably overexpressing of MTA1 or knockdown of MTA3. E RT-qPCR and western blotting to determine the mRNA and protein expression levels of stem cell markers (SOX2, OCT-4, c-MYC, and NANOG) after knockdown or overexpression of MTA1 or MTA3. Error bars represent the mean ± SD in A–E. *p < 0.05, **p < 0.01; two-tailed unpaired t-test.

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