Fig. 5: TRIM21 suppresses proliferation, invasion, EMT and stemness of breast cancer cells. | Cell Death & Disease

Fig. 5: TRIM21 suppresses proliferation, invasion, EMT and stemness of breast cancer cells.

From: The feedback loop between MTA1 and MTA3/TRIM21 modulates stemness of breast cancer in response to estrogen

Fig. 5

A Cell counting assays indicated that TRIM21 inhibits breast cancer cell growth in MCF-7 cells. Knockdown of TRIM21 actives breast cancer cell growth. B EdU assays performed on MCF-7 and MDA-MB-231 cells transfected with TRIM21, siCtrl, siTRIM21-1, or siTRIM21-2. siCtrl, siControl. C Transwell assays indicated that TRIM21 inhibits invasion in MCF-7 and MDA-MB-231 cells with overexpression it and actives invasion in TRIM21 knockdown cells. siCtrl, siControl. D Western blot results for the expression of epithelial and mesenchymal markers after overexpression or knockdown of TRIM21 in MCF-7 and MDA-MB-231 cells. E Percentage of EpCAM-positive cells assessed by flow cytometry were increased after TRIM21 knockdown in MDA-MB-231 cells. F Protein expression levels of the stemness markers SOX2, OCT4, NANOG, and c-MYC in MCF-7 and MDA-MB-231 cells overexpression or knockdown of TRIM21. G RFS and OS analyses demonstrated that breast cancer patients with high TRIM21 expression had a better prognosis than those with low expression in. H Proliferative ability of MDA-MB-231 cells transfected with siRNA and shRNA viruses determined using the EdU assays. I The invasive phenotype was detected in MCF-7 cells transfected with the Vector, MTA1, MTA3, and TRIM21. J Transwell assays to identify the invasiveness of MDA-MB-231 cells transfected with siRNA and shRNA viruses. Error bars represent the mean ± SD in A–C, E, H–J. *p < 0.05, **p < 0.01; two-tailed unpaired t-test.

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