Fig. 5: HIF1A-AS2 sponges miR-141-3p to downregulate its expression. | Cell Death & Disease

Fig. 5: HIF1A-AS2 sponges miR-141-3p to downregulate its expression.

From: HIF1A-AS2 promotes the metabolic reprogramming and progression of colorectal cancer via miR-141-3p/FOXC1 axis

Fig. 5: HIF1A-AS2 sponges miR-141-3p to downregulate its expression.

A Predicted miRNAs that have the potential to bind to HIF1A-AS2. B The binding efficiency of HIF1A-AS2 probe and control probe on HIF1A-AS2. C, D The binding efficiency of HIF1A-AS2 probe and control probe on candidate miRNAs. E FISH assay showing the subcellular location of miR-141-3p in CRC cells. Scale bar = 25 μm. F HIF1A-AS2 level in CRC cells transfected with miR-141-3p mimics or inhibitor. G RIP-qPCR assay was used to determine the binding effect between miR-141-3p and Ago2. H RIP-qPCR assay was used to determine the binding effect between HIF1A-AS2 and Ago2. I Sequences showing the predicted binding site of HIF1A-AS2 and miR-141-3p and relevant mutant sequences of HIF1A-AS2. J Luciferase activity of CRC cells transfected with wild type or mutant HIF1A-AS2 plasmid. K Kaplan–Meier curve comparing the OS between miR-141-3p-high group and miR-141-3p -low group in the FUSCC cohort. L Correlation between miR-141-3p and HIF1A-AS2 in 74 CRC samples in FUSCC cohort. *P < 0.05; **P < 0.01; ***P < 0.001; ns no significance.

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