Fig. 6: miR-141-3p binds to the 3’UTR of FOXC1 to downregulate its expression. | Cell Death & Disease

Fig. 6: miR-141-3p binds to the 3’UTR of FOXC1 to downregulate its expression.

From: HIF1A-AS2 promotes the metabolic reprogramming and progression of colorectal cancer via miR-141-3p/FOXC1 axis

Fig. 6: miR-141-3p binds to the 3’UTR of FOXC1 to downregulate its expression.

A Veen plot displaying the predicted mRNAs by four datasets. B Kaplan–Meier curve comparing the OS between FOXC1-high group and FOXC1-low group in TCGA cohort. C RT-qPCR showing the expression of FOXC1 after transfecting CRC cells with miR-141-3p mimics. D RT-qPCR showing the expression of FOXC1 after transfecting CRC cells with miR-141-3p inhibitors. E Western blot shows the expression of FOXC1 after transfecting CRC cells with miR-141-3p mimics or inhibitor. F Sequences showing the predicted binding site between the 3’UTR of FOXC1 and miR-141-3p and relevant mutant sequences of the 3’UTR of FOXC1. G Luciferase activity of CRC cells transfected with wild type or mutant FOXC1 3’UTR plasmid. H Relative FOXC1 mRNA levels of normal and tumor samples in TCGA cohort. I Correlation between miR-141-3p and FOXC1 mRNA level in TCGA cohort. J Kaplan–Meier curve comparing the OS between the FOXC1-high group and FOXC1-low group in the FUSCC cohort. K Correlation between miR-141-3p and FOXC1 in 74 CRC samples in the FUSCC cohort. *P < 0.05; **P < 0.01; ***P < 0.001; ns no significance.

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