Fig. 8: SP1 transcriptionally activates HIF1A-AS2 in CRC. | Cell Death & Disease

Fig. 8: SP1 transcriptionally activates HIF1A-AS2 in CRC.

From: HIF1A-AS2 promotes the metabolic reprogramming and progression of colorectal cancer via miR-141-3p/FOXC1 axis

Fig. 8: SP1 transcriptionally activates HIF1A-AS2 in CRC.

A The fragments of HIF1A-AS2 promoter were cloned into the PGL3 plasmid. B, C Relative luciferase activity of CRC cells transfected with the relevant fragment of HIF1A-AS2 promoter. D, E Luciferase activity assay showing the transcriptional activity of HIF1A-AS2 promoter in CRC cells with indicated treatments. F Relative HIF1A-AS2 level of CRC cells with different treatments in DLD1 cells. G Relative HIF1A-AS2 level of CRC cells with different treatments in HCT15 cells. H Predicted binding site of SP1 on HIF1A-AS2 and relevant mutant sequence. I Relative luciferase activity of CRC cells transfected with wild-type or mutant HIF1A-AS2 promoter. J CHIP-qPCR displaying the interaction of SP1 and HIF1A-AS2 promoter. K Representative IHC images showing SP1 expression in normal and tumor tissues. Scale bar = 200 μm (upper part), 50 μm (lower part). L SP1 scores of normal and tumor tissues in TMA cohort. M Correlation between SP1 score and HIF1A-AS2 score in TMA cohort. N, O Relative HIF1A-AS2 expression in CRC cells treated with different concentrations of lactate. P Relative HIF1A-AS2 expression in CRC cells with relevant treatments. Q Western blot showing SP1 expression in CRC cells with relevant treatments. *P < 0.05; **P < 0.01; ***P < 0.001; ns no significance.

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