Fig. 4: Induction of Zfp36 (TTP) by Ifnar1-signaling regulates TNFα expression and necroptosis. | Cell Death & Disease

Fig. 4: Induction of Zfp36 (TTP) by Ifnar1-signaling regulates TNFα expression and necroptosis.

From: Regulation of Zfp36 by ISGF3 and MK2 restricts the expression of inflammatory cytokines during necroptosis stimulation

Fig. 4

A Expression of TTP was analyzed by western blotting of cell extracts collected from WT and Ifnar1−/− BMDMs at various time intervals following treatment with LPS (1 ng/mL) and/or EMR (10 µM). B–E Expression of TNFα, IFNβ, and IL-6 was measured in the supernatants collected at different time intervals after the treatment of WT and Zfp36−/− BMDMs with LPS (1 ng/mL) (B), LPS (1 ng/mL)+EMR (10 µM) (C), IFNβ (10 ng/mL) (D), or IFNβ (10 ng/mL)+EMR (10 µM) (E). F, G Cell death was evaluated by staining of cells with Hoechst and PI at 24 h post treatment with LPS + /−EMR (F, G), IFNβ + /−EMR (F, H), TNFα ± EMR (F, I). Each experiment was repeated at least three times. (***P < 0.001, ****P < 0.0001).

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