Fig. 3: Prolonged survival of nude mice transplanted with GSPT1-KO U87 cells.
From: Potential of GSPT1 as a novel target for glioblastoma therapy
A GSPT1-KO U87 glioblastoma cells were generated by genome editing. Heterozygous (A-2) and homozygous (A-7) GSPT1-KO U87 cells were confirmed by immunoblotting with GSPT1 antibody. Comparative loading of proteins was confirmed by immunoblotting using GAPDH antibody. B Growth rates of the three cell lines were evaluated and plotted. n = 19 (WT), 4 (A-2), and 14 [KO (A-7)]. ****P < 0.0001 between WT and KO (A-7) (at 72 and 96 h) and WT and A-2 (96 h) by two-way ANOVA with Tukey’s post-hoc test. No significant differences were observed between A-2 vs A-7. C Experimental chart: 1 × 106 WT and GSPT1-KO (A-7) U87 cells were transplanted into the brains of nude mice. D Brain tumors transplanted with WT or GSPT1-KO U87 cells were removed, fixed, embedded in paraffin on day 20, stained with hematoxylin and eosin (HE), and immunostained for GSPT1 (n = 3). Scale bars, 100 μm. E The survival period from transplantation to death (x-axis) and survival rate (y-axis) were plotted. n = 3 (WT) and 5 (GSPT1-KO). **P = 0.0042 by the Kaplan–Meier method and log-rank test.
