Fig. 4: Cancelled prolonged survival of nude mice transplanted with Rescued GSPT1-KO U87 cells.
From: Potential of GSPT1 as a novel target for glioblastoma therapy
A GSPT1-KO (A-7) with stable overexpression of GSPT1-FLAG (Rescued GSPT1-KO) U87 glioblastoma cells were generated. Rescued and comparable expression of GSPT1-FLAG in Rescued GSPT1 U87 cells, compared to that in WT U87 cells, was confirmed by immunoblotting using GSPT1 antibody. Comparative loading of proteins was confirmed by immunoblotting using β-actin antibody. B Growth rates of the three cell lines were evaluated and plotted (n = 10 in WT and Rescued GSPT1-KO). No significant difference was observed between WT and Rescued GSPT1-KO U87 cells by two-way ANOVA with Tukey’s post-hoc test. Growth curve of GSPT1-KO(A-7) cells is shown as a reference. C Experimental chart: 1 × 106 GSPT1-KO as well as Rescued GSPT1-KO U87 cells were transplanted into the brains of nude mice. D Brain tumors transplanted with GSPT1-KO or Rescued GSPT1-KO U87 cells were removed, fixed, embedded in paraffin on day 20, and immunostained for GSPT1 (n = 5). Scale bars: 100 μm. E Survival period from transplantation to death (x-axis) and survival rate (y-axis) were plotted. n = 5; **P = 0.0018 by the Kaplan–Meier method and log-rank test.
