Fig. 6: GSPT1 expression in glioblastoma samples of patients, and the relationship between expression levels and patient prognosis.
From: Potential of GSPT1 as a novel target for glioblastoma therapy
A–C GSPT1 protein expression in three glioblastoma samples (Patient-1, Patient-2, and Patient-3) from 18 patients (data of the other 15 patients are presented in Fig. S3). Upper panels: gadolinium-enhanced MR images. Middle and lower panels show microphotographs of GSPT1 immunostaining with hematoxylin counterstaining. Patient-1 was a 60-year-old man with a right medial frontal lobe tumor. Patient-2 was a 64-year-old man with a tumor in the left frontal lobe. Patient-3 was a 59-year-old man with a right parietal lobe tumor. GSPT1 is expressed in the cytoplasm of glioblastoma cells. Notably, GSPT1 is not expressed in vascular cells. Patients-1 and -2 showed strong GSPT1 expression; however, Patient-3 showed weak to moderate GSPT1 expression. The numbers at the bottom left of each microphotograph represent the relative expression values of GSPT1 mRNA in real-time RT-PCR analyses. There was no association between GSPT1 immunostaining levels and relative mRNA expression values. Scale bars, 100 μm (upper panels) and 50 μm (lower panels). D Comparison of Kaplan–Meier curves of patients with glioblastoma according to the mRNA expression levels of GSPT1 in glioblastoma samples. Eighty-seven patients with glioblastoma were divided into two groups based on the median value of GSPT1 expression. GSPT1high: GSPT1 expression values were higher than the median values. GSPT1low: GSPT1 expression values were lower than the median values. Left graph: Dataset from Kobe University Hospital. Right graph: Dataset from TCGA Glioblastoma 2013 (https://www.cbioportal.org/), which are freely available.
