Fig. 1: Sorafenib-induced and DDX5-repressed genes enriched in Wnt/β-catenin and non-canonical NF-κB signaling.

A Heatmap of common genes between SOR-induced and DDX5-repressed genes, as described in [7]. B RT-PCR quantification of NIK mRNA using total RNA from HepAD38 and Huh7 cells transfected with control (siCtrl) or DDX5 siRNAs. DDX5 overexpression (DDX5^OE) using Dox-inducible-DDX5 cell lines grown with Dox (1.0 µg/ml) for 48 h and SOR for the last 24 h. C Immunoblots of NIK using lysates from HepAD38 and Huh7 cell lines transfected with siCtrl or siDDX5 ±10 µM SOR for 24 h. For DDX5^OE, Dox-inducible-DDX5 cell lines grown with Dox (1.0 µg/ml) for 48 h and SOR for the last 24 h. A representative immunoblot from three independent experiments (n = 3). Quantification shown in Supplementary Fig. S1. D RT-PCR quantification of NIK mRNA using total RNA isolated from Huh7 xenograft tumors, treated ± SOR, as indicated. Data expressed as mean ± SEM from eight tumors, described in Li et al. [7]. *p < 0.05 by unpaired t test. E, F qRT-PCR of NIK mRNA and immunoblots of NIK protein, using total RNA or lysates, respectively, isolated from WT and DDX5^KO Huh7 cells, ±SOR (10 µM) for 24 h, as indicated. E qRT-PCR data expressed as mean ± SEM from n = 3. *p < 0.05, **p < 0.01 by unpaired t test. F A representative NIK immunoblot from n = 3.