Fig. 2: DDX5 via Wnt activation regulates non-canonical NF-κB signaling in sorafenib treated cells. | Cell Death & Disease

Fig. 2: DDX5 via Wnt activation regulates non-canonical NF-κB signaling in sorafenib treated cells.

From: DDX5 deficiency drives non-canonical NF-κB activation and NRF2 expression, influencing sorafenib response and hepatocellular carcinoma progression

Fig. 2

A WT and DDX5KD Huh7 and HepAD38 cells co-transfected with MAP3K14-firefly luciferase and Renilla-luciferase (100 ng each plasmid per 12-well plate) ±SOR (10 µM), XAV939 (20 µM) or siβ-catenin (50 pM), as indicated. Data expressed as mean ± SEM, n = 3. *p < 0.05, ** p < 0.01 by unpaired t test. B qRT-PCR of NIK mRNA using total RNA from WT and DDX5KD HepAD38 transfected with siβ-catenin (50 pM) and treated ± SOR (10 µM). Data expressed as mean ± SEM, n = 3. *p < 0.05, ** p < 0.01 by unpaired t test. C Huh7 WT and DDX5KO cells co-transfected with Wnt-reporter (TopFlash) and Renilla-luciferase (100 ng each plasmid per 12-well plate), and siRNAs (50 pM each) siCtrl or siβ-catenin, ±SOR (10 µM) for 24 h. Data expressed as mean ± SEM, n = 3. **p < 0.01 and ***p < 0.001 by unpaired t test. D qRT-PCR of NIK mRNA using total RNA from WT and DDX5KO Huh7 cells, transfected with siβ-catenin (50 pM), and treated ±SOR (10 µM), as indicated. Data expressed as mean ± SEM, n = 3. *p < 0.05, ** p < 0.01 ***p < 0.001 by unpaired t-test.

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