Fig. 3: UBE2M inhibited the ubiquitination and degradation of ERα via UBE2M-CUL3/4A-E6AP-ERα axis. | Cell Death & Disease

Fig. 3: UBE2M inhibited the ubiquitination and degradation of ERα via UBE2M-CUL3/4A-E6AP-ERα axis.

From: UBE2M forms a positive feedback loop with estrogen receptor to drive breast cancer progression and drug resistance

Fig. 3

A UBE2M silencing inhibited the expression of ERα. B UBE2M silencing shortened the half-life of ERα protein. C The ERα silencing-induced decrease in UBE2M expression was reversed by the proteasome inhibitor MG132. D UBE2M silencing promoted the polyubiquitination of ERα. E UBE2M silencing promoted the interaction between ERα and E6AP. F UBE2M silencing enhanced the expression of E6AP. G CUL3 or CUL4A silencing led to elevated levels of E6AP and decreased levels of ERα. H CUL3 or CUL4A silencing led to an extended half-life of the E6AP protein and a shortened half-life of the ERα protein. I UBE2M silencing effectively inhibited neddylation levels of both CUL3 and CUL4A. Data are presented as mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001 by two-tailed unpaired t-test.

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