Fig. 4: UBE2M silencing inhibited cell growth by inducing cell cycle arrest and apoptosis. | Cell Death & Disease

Fig. 4: UBE2M silencing inhibited cell growth by inducing cell cycle arrest and apoptosis.

From: UBE2M forms a positive feedback loop with estrogen receptor to drive breast cancer progression and drug resistance

Fig. 4

A Two sgRNA-UBE2M oligos were used to silence UBE2M in MCF7 and T47D cells using the CRISPR/Cas9 system. B–D UBE2M silencing inhibited the growth of both MCF7 and T47D cells. E UBE2M silencing inhibited the colony-forming ability of both MCF7 and T47D cells. F, G UBE2M silencing induced cell cycle arrest. PI staining and FACS analysis were used to analyze the cell cycle profile upon UBE2M silencing (F). Western blotting was used to assess the expression of cell cycle-related proteins, namely, p21, p27 and WEE1 (G). H, I UBE2M silencing induced cell apoptosis. AnnexinV-FITC/PI double-staining analysis was used to analyze the cell apoptosis upon UBE2M silencing (H). Western blotting was used to assess the expression of the apoptosis-related proteins BIM (BIMEL, BIML and BIMS), NOXA and cleaved PARP (C-PARP) (I). Data are presented as mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001 by two-tailed unpaired t-test.

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