Fig. 5: UBE2M silencing sensitised ER-positive breast cancer cells to fulvestrant in vitro.

A, B UBE2M silencing and fulvestrant synergically inhibited the expression of ERα and UBE2M. C, D UBE2M silencing and fulvestrant synergically inhibited cell growth. The cell growth was determined via ATP-lite assay. E UBE2M silencing and fulvestrant synergically inhibited the colony-forming ability of breast cancer cells. The colony-forming ability was determined via colony formation assay. Data are presented as mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001 by two-tailed unpaired t-test.