Fig. 2: PA elicits GSDME-dependent pyroptosis in ATC cells. | Cell Death & Disease

Fig. 2: PA elicits GSDME-dependent pyroptosis in ATC cells.

From: Prosapogenin A induces GSDME-dependent pyroptosis of anaplastic thyroid cancer through vacuolar ATPase activation-mediated lysosomal over-acidification

Fig. 2

A Morphologic alterations of 8505C, KHM-5M, C643, BHT-101and HTh-7 cells induced by PA (5 μM) (scale bar: 20 μm). B 8505C and KHM-5M cells were treated with PA (5 μM) for 24 h and processed for electron microscopy, (scale bar: 10 μm). C After PA (0, 4, 5 and 6 μM) treatment for 24 h, relative LDH activity in culture mediums of 8505C and KHM-5M cells were detected by the LDH assay kit, D and the GAPDH, full-length GSDME and GSDME-N terminus protein levels were measured by western blot. E After knockdown (KD) GSDME and GSDMD, 8505C and KHM-5M cells were treated with PA (5 μM), morphologies were photographed by a phase contrast microscopy (scale bar: 20 μm). F After GSDME-KD, 8505C and KHM-5M cells were treated with PA (5 μM) for 24 h, cell death rates were assessed by flow cytometry, and G LDH activitiy in culture mediums was detected by the LDH assay kit. Data are shown as mean ± SD for n = 3 (biological replicates). **p < 0.01, ***p < 0.001.

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