Fig. 2: Crizotinib induced ferroptosis in AML12 cells (n = 3). | Cell Death & Disease

Fig. 2: Crizotinib induced ferroptosis in AML12 cells (n = 3).

From: The involvement of the Stat1/Nrf2 pathway in exacerbating Crizotinib-induced liver injury: implications for ferroptosis

Fig. 2: Crizotinib induced ferroptosis in AML12 cells (n = 3).

A Signification enriched top 20 KEGG pathway for the DEGs between the control and Crizotinib group. B and C The changes of ACSL4, SLC7A11, GPX4 protein expression (B), and PTGS2 mRNA levels (C) in AML12 cells treated with Crizotinib in different concentrations for 48 h were observed. D and E Iron content (D) and MDA (E) were measured by reagent kit following treatment with increasing concentrations of Crizotinib for 48 h. F The induction of lipid ROS was determined by BODIPY™ 581/591 C11 and flow cytometry for 48 h. G Representative TEM images showed mitochondrial damages treated with 13 μM Crizotinib for 48 h in the AML12 cell. Scale bars, 2 μm (upper panel) and 500 nm (lower panel). *P < 0.05, **P < 0.01 and ***P < 0.001 vs. control group. Crizo Crizotinib.

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