Fig. 2: TCP1 alters the lipid composition of GCB DLBCL. | Cell Death & Disease

Fig. 2: TCP1 alters the lipid composition of GCB DLBCL.

From: TCP1 expression alters the ferroptosis sensitivity of diffuse large B-cell lymphoma subtypes by stabilising ACSL4 and influences patient prognosis

Fig. 2

A, B ROS levels of RSL3-treated TCP1-knockdown DB and SU-DHL-4 cells. C, D Lipid peroxidation levels in RSL3-treated TCP1-overexpressing DB and SU-DHL-4 cells measured using C11-BODIPY (581/591) staining. E, F MDA activity of RSL3-treated TCP1-knockdown and TCP1-overexpressing DB and SU-DHL-4 cells. G, H Representative images of Phen Green SK staining in RSL3-treated TCP1-overexpressing DB and SU-DHL-4 cells as observed using confocal laser scanning microscopy (CLSM) (scale bars: 50 μm). I TEM images showing the mitochondrial morphology of the control and TCP1-knockdown groups following RSL3 treatment (24 h). The control group shows increased mitochondrial membrane density and shrinkage, whereas the TCP1-knockdown group shows no significant changes in mitochondrial morphology (black arrows). Scale bar: 2 μm. J Heatmap of lipid composition differences in RSL3-treated TCP1-knockdown DB cells. K Heatmap of lipid composition differences in TCP1-knockdown DB cells. L, M Relative content of PE-18:0/22:4 and PE-18:0/20:4 in RSL3-treated DB cells of the empty vector and TCP1-knockdown groups compared with the untreated group. Data are presented as mean ± SD; *p < 0.05, **p < 0.01, ***p < 0.001.

Back to article page