Fig. 2: Expression analysis of miR-29a-3p and its downstream molecules.
From: Circ-0044539 promotes lymph node metastasis of hepatocellular carcinoma through exosomal-miR-29a-3p
A The localization of miR-29a-3p in HCC, data from RNALocate (http://www.rna-society.org/rnalocate). B FISH showed the localization of circ-0044539 (green) and miR-29a-3p (pink) in HCC cell lines. Nuclei (DAPI) are shown in blue. Scale bar, 50 µm. C The location of circ-0044539 in HCC-LM6 cells was identified by subcellular fractionation assay and qRT-PCR (n = 3). MALAT1 and β-actin were used as the nuclear and cytoplasm controls, respectively. D The expression of miR-29a-3p in paired tumor and adjacent normal tissues in the indicated tumor types from the TCGA database, using Wilcoxon signed rank test. E, F miR-29a-3p expression in different N stages in BRCA (E) and PRAD (F). G STRING protein interaction analysis showed the downstream targets of miR-29a-3p. H Signaling pathway showed VEGFA-PI3K-Akt-mTOR-HIF-1α from the WikiPathway database. I The starBase database presented the binding site of miR-29a-3p on the VEGFA 3’UTR. J, K The expression levels of miR-29a-3p (J) and VEGFA (K) in HCC tissues and normal liver tissues. L The correlation between HIF-1α and CXCR4, data from the TCGA LIHC cohort was assessed with Spearman analysis. *p < 0.05, **p < 0.01, ***p < 0.001.
