Fig. 6: Increased immunosuppression activity of PMN-MDSCs following treatment with exosomes derived from HCC.
From: Circ-0044539 promotes lymph node metastasis of hepatocellular carcinoma through exosomal-miR-29a-3p
A Experimental schematic diagram of PMN-MDSCs, sorted from HCC patients and then co-cultured with HCC exosomes, cultured with CD3 + CD8 + T cells isolated from healthy donors to evaluate PMN-MDSC function. B The Arginase mRNA expression level and Arginase activity in PMN-MDSCs with the indicated HCC-exosome treatments were assessed. C, D The ROS and NO levels in PMN-MDSCs with indicated treatments were detected. E, F The CFSE staining assay showed T-cell proliferation. Representative fluorescence diagram (E) and flow cytometric data (F) are shown. The percentage of the CFSE low population represents the proportion of proliferating CD3 + CD8 + T cells. The proliferation index and the divided index were calculated. G LDH release assays of T cells co-cultured with the indicated PMN-MDSCs were detected. H A schematic illustration of the molecular mechanism through which circ-0044539-derived exosomal miR-29a-3p regulates PMN-MDSC immunosuppression ability. Data presented as means ± SD of three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 (Student’s t-test).
