Fig. 2: POLRMT overexpression in CRC tissues and cells.
From: Targeting POLRMT by IMT1 inhibits colorectal cancer cell growth
POLRMT mRNA and protein levels were evaluated in colon cancer tissues (“T”) and corresponding adjacent normal colon tissues (“N”) obtained from a cohort of twenty primary colon cancer patients (A–C). The expression of POLRMT mRNA and protein was assessed in listed primary human colon cancer cells (“pCan1”/”pCan2”) and primary human colon epithelial cells (“pEpi1”/”pEpi2”) (D, E). “pEpi1”/”pEpi2” cells were subjected to treatment with IMT1 (1 μM) for 72 h, followed by assessments of cell viability using CCK-8 (F) and determination of cell death through Trypan blue staining (G). The data were presented as mean ± standard deviation (SD, n = 5), with *indicating P < 0.05 compared to “N” tissues (A, C) or “pEpi1” (D, E). *Indicating P < 0.05 compared to “Veh” treatment (G) and “n. s.” non-statistical difference (P > 0.05) (F, G). The experiments were repeated five times, yielding consistent results.
