Fig. 4: CCDC113 knockdown inhibits CRC tumorigenesis and metastasis in vivo.
From: CCDC113 promotes colorectal cancer tumorigenesis and metastasis via TGF-β signaling pathway
A, B CCDC113 knockdown HCT116 cells and control cells were subcutaneously injected into BALB/c nude mice for 24 days. Representative images of subcutaneous xenograft tumors were shown in left panel. Statistical results of tumor volumes (A) and tumor weights (B) were shown in right panel (n = 4 per group). C CCDC113 expression was detected by Western blotting in shNC and shCCDC113 of subcutaneous xenograft tumors. D, E IHC staining detected CCDC113 expression (D) and Ki67 expression (E) in subcutaneous xenograft tumors (n = 4 per group). Scale bars, 50 μm. F CCDC113 knockdown HCT116 cells and control cells were injected into tail vein of BALB/c nude mice for 42 days. Representative images of liver metastasis nodules were shown in left panel and statistical results were shown in right panel (n = 3 per group). G Liver metastasis nodules in (F) were analyzed by HE staining. Representative images were shown in left panel and statistical results were shown in right panel (n = 3 per group). Scale bars, 1 mm. H IHC staining detected CCDC113 and Ki67 expression in liver metastasis tissues (n = 3 per group). Scale bars, 50 μm. Data are presented as means ± SD. ***p < 0.001, **p < 0.01, *p < 0.05.
