Fig. 4: The role of PD-L2 in SPHK1-elicited cell migration and invasion of bladder cancer.
From: SPHK1 promotes bladder cancer metastasis via PD-L2/c-Src/FAK signaling cascade
A GSEA analysis of immune regulation and adhesion-related pathway using bladder cancer samples from TCGA database (high SPHK1 group = 117; low SPHK1 group = 282). B The profile of CD274 (PD-L1) or PDCD1LG2 (PD-L2) expression in different subtypes of bladder cancer from the TCGA database analyzed by one-way ANOVA. C The correlation of PD-L1 or PD-L2 with SPHK1 gene expression in bladder cancer samples from TCGA database. D The profile of PD-L1 and PD-L2 mRNA and protein expression in 253J-BV bladder cancer cell lines (n = 3). E, F The regulation of PD-L2 mRNA and protein expression by SPHK1 activities in bladder cancer cell lines cells (n = 3). G, H The effect of PD-L2 on cell migration and invasion of 253J-BV PD-L2 KO cells and 253J PD-L2 OE (n = 3). All results were presented as mean ± SD and statistically calculated (*p < 0.05, #p < 0.05, **p < 0.01, ##p < 0.01, ***p < 0.001, ****p < 0.0001).
