Fig. 3: CARM1 enhances malignant behaviors of gastric cancer cells under low glucose condition.
A Proliferation of MGC-803 and AGS cells with parental CARM1 (Lenti-V2) or stable CARM1 knockdown (CARM1-sg) treated with 2.5 mmol/L glucose (Low Glc) was determined by cell counting. B, C Colony formation assay of MGC-803 and AGS cells with Lenti-V2 or CARM1-sg treated with 2.5 mmol/L glucose. D MGC-803 and AGS cells with Lenti-V2 or CARM1-sg treated with 2.5 mmol/L glucose were harvested, fixed, and stained with propidium iodide for DNA content analysis using flow cytometry. Cell ModFit software was used to calculate the percentages of cells in the G1, S, and G2/M phases. E MGC-803 and AGS cells with Lenti-V2 or CARM1-sg were cultured in 6-well plates and treated with 2.5 mmol/L glucose for 18 h. Subsequently, the cells were exposed to EdU for 2 h and then stained with Apollo reaction cocktail for 30 min. Fluorescence microscopy was used to examine the EdU-labeled replicating cells. F Percentage of cells undergoing apoptosis, normalized against the total cell count in MGC-803 and AGS cells with Lenti-V2 or CARM1-sg under low glucose conditions. Cell apoptosis measured by Annexin-V/propidium iodide assays. G, H Dissected tumors from a xenograft mouse model with transplanted MGC-803 cells with Lenti-V2 or CARM1-sg (G). Volumes and weights of tumors on day 30 (H). Data are shown as mean ± SEM. Values of P < 0.05 considered statistically significant. *P < 0.05, **P < 0.01, ***P < 0.001.
