Fig. 5: SMg modulates TAM density, spatial distribution and function.

A Two-photon images at D21 and D28 of EGFP⁺ cells (green), EYFP⁺ cells (yellow) and EGFP⁺/ EYFP⁺ cells (blue). Tumors are outlined in red (scale bar = 300 µm). Representative images are shown. B Quantification of EGFP⁺, EYFP⁺ and EGFP⁺/EYFP⁺ cell densities in vehicle (n = 9) and SMg (n = 3) treated tumors. Statistical analyses were performed by using the Mann-Whitney test; alpha = 0.05, bilateral p-value: *p < 0.05; **p < 0.005; ***p < 0.0005; ****p < 0.0001). C Violin Plot representing the distance of the EGFP⁺, EYFP⁺ and EGFP⁺/EYFP⁺ cells from vessels at D21 and D28 for each condition. Each dot represents a cell with a distance from tumor vessels expressed in µm. Statistical analyses were performed by using un-paired t-test; alpha = 0.05, bilateral p-value: **p < 0.005; ***p < 0.0005; ****p < 0.0001. D Confocal images of EGFP⁺ (green), EYFP⁺ (yellow), MHC-II⁺ (white) cells and GL261-DsRed tumor cells (red) (scale bar = 20 µm). Representative images of D28 tumors are shown. E Dot plot representing the number of cells expressing EGFP⁺, EYFP⁺, EGFP⁺/EYFP⁺ and co-expressing MHC-II in vehicle (n = 6) and SMg-treated (n = 6) tumors at D28. Statistical analyses were performed by using Mann–Whitney test; alpha=0.05, bilateral p-value: *=< 0.05; **p < 0.005. A 3D reconstitution and image analyses were performed by using Imaris software. B, E Bar graphs represent mean ± s.e.m.