Fig. 1: CircSEC24B was upregulated in CRC cells and tissues. | Cell Death & Disease

Fig. 1: CircSEC24B was upregulated in CRC cells and tissues.

From: CircSEC24B activates autophagy and induces chemoresistance of colorectal cancer via OTUB1-mediated deubiquitination of SRPX2

Fig. 1

A Top 10 differentially expressed circRNAs between RAPA-treated and NC group were visualized by Volcano plot. B RT-PCR assay for the top 10 circRNAs with divergent primers. C The backsplice junction site of circSEC24B was determined via Sanger sequencing. D RT-PCR assay with cDNA and genomic DNA using circSEC24B divergent and convergent primers. E RT-qPCR assays for circSEC24B with the NCM460 and the five CRC cell lines. F RT-qPCR assays for circSEC24B by CRC tissue and normal tissue samples. G RT-qPCR assay for circSEC24B, SEC24B GAPDH with or without RNAse R treatment. H RNA-FISH for circSEC24B with or without treatment of RNAse R, DAPI (blue) was used for nuclei staining. I RT-qPCR assay for circSEC24B expression with various dose of RAPA in HCT116 cell line. J RT-qPCR assay for circSEC24B in OXA-resistant or OXA-nonresistant LoVo and HCT116 cell lines. K RNA-FISH assay for circSEC24B in HCT116 and HCT116/OXA cell lines.

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