Fig. 4: CircSEC24B promoted the expression of SRPX2 in CRC cells. | Cell Death & Disease

Fig. 4: CircSEC24B promoted the expression of SRPX2 in CRC cells.

From: CircSEC24B activates autophagy and induces chemoresistance of colorectal cancer via OTUB1-mediated deubiquitination of SRPX2

Fig. 4

A RT-qPCR assay for SEC24B mRNA with a stable transfection of circSEC24B or sh-circSEC24B in LoVo/OXA and HCT116/OXA cells. B The argonaute 2 (Ago2) cross-linking immunoprecipitation to assess the correlation between circSEC24B and AGO2 in LoVo/OXA and HCT116/OXA cells. C The differentially expressed proteins induced by shCircSEC24B or RAPA were intersected with DEGs of GSE42387 sets. D RT-qPCR assay for the mRNA expression level of the selected five genes with circSEC24B overexpression or knockdown. E Western blot assay for DLG4, SRPX2, BCL2L1, SGK1, and ABTB2 with upregulation or downregulation of circSEC24B in LoVo/OXA and HCT116/OXA cells. F Biotin-labeled RNA pull-down assay for evaluation of the interplay between circSEC24B and SRPX2 in LoVo/OXA and HCT116/OXA cell line. G RIP assay was used to assess the endogenous enrichment of circSEC24B with SRPX2 antibody in HCT116/OXA cells. H, I Dual RNA-FISH and immunofluorescence assays to the localization of circSEC24B and SRPX2 with upregulation or downregulation of circSEC24B in LoVo/OXA and HCT116/OXA cells.

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