Fig. 8: OTUB1 regulates SRPX2 in an acetylation-dependent approach. | Cell Death & Disease

Fig. 8: OTUB1 regulates SRPX2 in an acetylation-dependent approach.

From: CircSEC24B activates autophagy and induces chemoresistance of colorectal cancer via OTUB1-mediated deubiquitination of SRPX2

Fig. 8

A Western blot assay for SRPX2 protein in LoVo/OXA and HCT116/OXA cells with NAM at different points. B Western blot assay for SRPX2 acetylation levels in LoVo/OXA and HCT116/OXA cells with NAM at different points. The LoVo/OXA and HCT116/OXA cells were transfected with SRPX2-FLAG. C RT-qPCR assay for SRPX2 mRNA levels in LoVo/OXA and HCT116/OXA cells treated with NAM at different points. D Co-IP assay was used to evaluated the effect of NAM on binding degree of SRPX2 to OTUB1. E Western blot assay was applied to evaluate whether NAM regulation of SRPX2 expression depends on the normal expression of OTUB1. F Western blot assay for SRPX2 protein level in HCT116/OXA cells treated with EX-527 at different points. G Co-IP assay was used to evaluated the effect of WT-SIRT1 or Mut-SIRT1 on SRPX2 acetylation levels. H Western blot assay was used to evaluated the effect of WT-SIRT1 or Mut-SIRT1 on SRPX2 protein levels.

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