Fig. 3: NKp46+ILC3 regulation in colitis requires GPX4 for pathogen resistance and barrier repair.
From: GPX4 restricts ferroptosis of NKp46+ILC3s to control intestinal inflammation
Gpx4fl/fl and RorccreGpx4fl/fl mice were orally inoculated with 1×108 CFU of C.R, and their tissues were collected on Day 8 post-infection. a Representative spleen images and b statistical results of spleen weights at Day 8 post-infection. (n = 3) c Representative H&E staining of the colon sections from indicated mice with C.R infection (scale bars: 100 μm). d The statistical results of histological scores are shown. (n = 4) e Body weight changes of indicated mice. (n = 4) Log10 CFU of C.R in f liver and g spleen tissues on day 8 post infection. (n = 3) h The representative flow cytometry plots of CD4+RORγt+ cells in LPMCs from the indicated mice. i Statistical results of proportion (left) and absolute numbers (right) of CD4+RORγt+ cells are shown. (n = 4) j Representative flow cytometry plots and statistical results of the proportion (left) and absolute number (right) of k ILC3s, including l NKp46+ILC3, m CCR6+ILC3, and n DN cell subsets in LPMCs of Gpx4fl/fl and RorccreGpx4fl/fl mice following C.R infection. (n = 4) o Representative FACS plots of IL-22- (upper) and IL-17A-positive (lower) ILC3s in LPMCs from the indicated mice. Statistical results of proportion (left) and absolute numbers (right) of p IL-22-, and q IL-17A-expressing ILC3s are shown. (n = 3) r Statistical results of the proportion (left) and numbers (right) of neutrophils in LPMCs from the indicated mice. (n = 4) s Relative RegIIIβ and RegIIIγ mRNA expression in the colon tissue of indicated mice. (n = 3) Statistical results of t the percentage of annexin V and 7-AAD double-negative population and u lipid ROS production in LPMC-derived ILC3s from the indicated mice. (n = 3) v Fe2+ level in ILC3s from LPMCs was analyzed using FerroOrange, and the statistical results are shown. (n = 3) Data are presented as the mean ± SEM or median, and statistical significance was determined using two-sided unpaired t-test (b, d–g, i, k–n, p–v). *P < 0.05; **P < 0.01; ***P < 0.001.
