Fig. 3: MSR signature enhanced by NAD supplementation in multiple cell models. | Cell Death & Disease

Fig. 3: MSR signature enhanced by NAD supplementation in multiple cell models.

From: NAD+-boosting agent nicotinamide mononucleotide potently improves mitochondria stress response in Alzheimer’s disease via ATF4-dependent mitochondrial UPR

Fig. 3

a–j Levels of UPRmt-related chaperone and protease proteins in N2a APPswe cells (n = 6 per group). At least three experiments were repeated independently with similar results. Data were analyzed by two-sided one-way ANOVA followed by Tukey’s multiple comparisons test. k Confocal images of HEK293 Tau P301L cells double immunostained with ATF4 (green) and mitochondria outer membrane protein TOM20 (red) antibody, after 24 h of NMN incubation. Scale bar, 10 μm. l–n 3D reconstruction images of HEK293 Tau P301L cells double immunostained with CHOP (green) and CYC (red) antibodies. Scale bar, 10 μm. o Relative levels of proteins implicated in the mitophagy pathway. p Quantification of the colocalization of the mitochondrial protein CYC and the autophagy protein LC3 in HEK293 Tau P301L cells after treatment with NMN. Scale bar, 10 μm. Immunoblot data were analyzed by two-sided one-way ANOVA followed by Tukey’s multiple comparisons test while the PCC were analyzed by two-sided unpaired t-test. q UPRmt transcript levels in N2a neuroblastoma cell line. All experiments were performed independently with at least three biological replicates. Data are shown as mean ± s.e.m. The p-values are indicated on the graphs. ns not significant.

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