Fig. 9: scRNA-seq identifies oligodendrocyte subclusters. | Cell Death & Disease

Fig. 9: scRNA-seq identifies oligodendrocyte subclusters.

From: NAD+-boosting agent nicotinamide mononucleotide potently improves mitochondria stress response in Alzheimer’s disease via ATF4-dependent mitochondrial UPR

Fig. 9

a Two-dimensional UMAP plots of re-clustered oligodendrocytes showing eight subclusters. Dots are colored based on the scRNA-seq clustering. b Heat map showing average gene expression of top DEGs in eight subclusters of oligodendrocytes. c Violin plots indicating average gene-expression across oligodendrocytes of mitophagy (pink1, binp3l, fkbp8) and UPRmt (atf4, atf5, hspd1, hspd9, yme1l1, eif2a) associated genes between 5xFAD versus NMN-treated 5xFAD mice. d Multiple volcano plots illustrating significant DEGs in oligodendrocyte subclusters in 5xFAD versus NMN-treated 5xFAD samples. e Violin plots showing expression of UPRmt genes in oligodendrocyte subclusters. f Representative immunofluorescence images and quantification of Olig2+ oligodendrocytes labeling with ATF4. The enlarged picture indicates Olig2+ oligodendrocyte was high colocalization with ATF4 in NMN-treated mice. The experiment was repeated twice independently. Scale bar, 25 μm (n = 3 mice; two-sided unpaired t-test). All experiments were performed independently with at least three biological replicates. Data are shown as mean ± s.e.m. The p-values are indicated on the graphs. ns not significant.

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