Fig. 5: DTL promoted metastasis of HCC cells in vitro and vivo. | Cell Death & Disease

Fig. 5: DTL promoted metastasis of HCC cells in vitro and vivo.

From: Hypoxia-induced DTL promotes the proliferation, metastasis, and sorafenib resistance of hepatocellular carcinoma through ubiquitin-mediated degradation of SLTM and subsequent Notch pathway activation

Fig. 5

A, B The effect of DTL overexpression and knockdown on Huh-7 and HCCLM-3 cells wound healing. C Transwell assays were performed to evaluate the migration ability of DTL overexpression and knockdown HCC cells. D Representative images of lung tissues in tail vein tumor metastasis mouse model from DTL overexpression and vector groups. E Representative images of liver tissues in orthotopic liver transplantation model from DTL overexpression and vector groups. F Representative images of lung metastatic nodules in hematoxylin–eosin (HE) stained sections. The left panel shows the number of lung nodules in DTL overexpression and vector groups from the tail vein tumor metastasis mouse model. G The relative liver weight was calculated as the ratio of wet liver weight to body weight ratio. H Representative images of intrahepatic metastasis nodules in HE‐stained sections. Left panel: the number of intrahepatic metastasis nodules in the DTL knockdown group. I Representative images of lung metastatic nodules in HE‐stained sections. Left panel: the number of lung nodules in DTL overexpression and vector groups from orthotopic liver transplantation model. *P < 0.05, **P < 0.01, ***P < 0.001, ns no significance.

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